| External factors: | Furanoflavones pongapin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Breast cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: | ANF's EC50 value for inhibition of MCF7-1A1 cell proliferation was determined to be > 20 μM. Nevertheless, the methanolic extract (EC50 for inhibition of proliferation of MCF7-1A1, 3.8 ± 0.4 μg/mL) from the seeds of P. pinnata, and the plant's secondary metabolites pongapin (EC50 for inhibition of MCF7-1A1 proliferation, 0.6 ± 0.15 μM), lanceolatin B (EC50 for inhibition of MCF7-1A1 proliferation, 0.7 ± 0.12 μM) do block MCF71A1 cells at G0-G1 and repress cyclin D1 levels , when cells were treated with 2× EC50 concentration of extract or metabolites.Interestingly, it was found that the P. pinnata seed-extract, pongapin and lanceolatin B do induce β-galactosidase in MCF7-1A1 cells. Approximately 60–70% cells were positive for β-galactosidase activity out of which more than 20% cells were strongly positive (cells staining dark blue). The control MCF7-1A1 cultures showed less than 10% cells positive for β-galactosidase and were weakly stained (cells stained light blue). |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: