Description: |
We observed that cells treated with 20 μM DhL for 48 h were larger and flatter than control cells, and a striking increase in the number of cells that stained positive for SA-β-Gal activity in situ (nearly 60%) was observed. For the simultaneous and therapeutic protocols, SA-β-Gal was of 40 picoM/g × 105 and 60 picoM/g × 105, respectively, in tumor supernatants of control mice, and for DhL mice SA-β-Gal activity was 80 picoM/g × 105 and 100 picoM/g × 105, respectively, indicating that DhL increased by 65% SA-β-Gal activity following both protocols. Also, the number of cells from dissected tumors that were positive for SA-β-Gal activity in situ increased. Following the simultaneous and therapeutic protocols, 5% and 8% of cells, respectively, were senescent in tumors of control mice, while in tumors of DhL mice, these values increased to 25 and 30%, respectively . |