External factors: | Protease inhibitors |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Lipodystrophic syndromes |
Experiment: | SA-β-gal activity assay//BrdU assay |
Description: | Strong cellular staining for 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) at pH 4, indicating physiological lysosomal β-galactosidase activity, was detected in control fibroblasts and in those bearing LMNA mutations or treated with PIs.LMNA mutations and PI treatment also induced a striking decrease in fibroblasts’ replicative capacity, measured in terms of bromodeoxyuridine (BrdU) incorporation in halfconfluent cells . |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: