| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//qRT-PCR//Western blot//DAPI staining//Flow cytometry |
| Description: | SA-β-gal activity assay: H2O2-treated NIH3T3 cells gradually became enlarged, flattened and were largely positive for SA-GLB1 staining from day 3. DAPI:The SAHFs in these cells were evident as large and irregularly shaped nuclear puncta. The arrest of cell growth was apparent,Western blot:and the levels of TRP53 protein. qRT-PCR:and Cdkn1a, and Il6 mRNA were elevated. Flow cytometry:H2O2 treatment also elevated intracellular ROS . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: