| External factors: | ROS |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | After treatment with hydrogen peroxide, the level of intracellular ROS was measured using H2DCFDA, which is converted to a fluorescent product, DCF, upon oxidation [37]. The ROS accumulation after hydrogen peroxide treatment in hUCB-MSCs was greater than that in human fibroblast and cancer cells.While the morphologies of the human fibroblast cells did not change significantly, hUCB-MSCs became larger and flatter after treatment with hydrogen peroxide. Staining cells with SA-β-Gal, a marker for detecting cellular senescence, revealed that hUCB-MSCs underwent senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: