| External factors: | 6-diazo-5-oxo-L-norleucine |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Exposure of both, early-passage (20 PDL) and late-passage (52 PDL) HUVEC cultures to DON strongly inhibited cell proliferation. Furthermore, exposure of early-passage HUVEC to the glutaminase inhibitor induced phenotypic alterations typically of premature senescence within two passages,The percentage of SA-β gal positive cells increased from 7.4 ± 1% to 25 ± 3% in young HUVEC exposed to 10 lM DON, and reached 66.0 ± 4% when 40 lM DON was used. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: