| External factors: | Doxorubicin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry//PI staining//ELISA |
| Description: | On the 13th day the CHEMO-treated cells exhibited several features of senescence: flatten morphology , increased size and granularity , augmented SA-β-gal activity and polyploidization. Moreover, the elevated expression of DDR proteins: γ-H2A.X, p-p53, and p21, and geroconversion markers [7]: cyclin D1 and p-S6 was detectable. In addition, the cells up-regulated secretion of SASP factors: VEGF and IL-8 . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: