| External factors: | 5-FU |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry//Cell activity assay//ELISA//PI staining |
| Description: | Treatment with DOXO and IRINO resulted in the marked increase of the size of cells and their nuclei, whereas no such effects were produced by OXA. Cells treated with 5-FU exhibited the intermediate morphological changes. All drugs induced growth arrest and the increase in cell granularity, as shown by SSC/FSC parameters measured by flow cytometry. It should be noted that treatment with DOXO and IRINO produced the largest HCT116cells . Moreover, the SA-β-galactosidase activity was the most pronounced in these cells. The fraction of SA-β-gal positive cells increased with every cycle of 5-FU treatment. Next, we analyzed the cell cycle distribution using PI staining.Following DOXO or IRINO treatment, the percentages of diploid cells in G0/G1 phase of the cell cycle were significantly reduced . The DOXO treatment decreased also fractions of diploid cells in the G2/M phase and/ or tetraploid cells arrested in the G0/G1 phase.Finally, DOXO or IRINO treatments produced the strongest increase in the fraction of polyploid cells. We found that 5-FU, DOXO, or IRINO treatment led to strong and prolonged accumulation of the cell cycle inhibitor - p21 and the geroconversion marker - CYCLIN D1 ,we showed that repeated cycles of IRINO and especially DOXO led to the marked augmentation of VEGF and IL-8 secretion. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: