| External factors: | Purple sweet potato color |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Cardiovascular disease |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | In the HFD+PSPC group, the area of SA β-gal-positive region in vascular vessels was markedly decreased,PSPC treatment significantly increased the expression of SMP30 in the endothelium, similar to that in the control group,in which strong immunoreactivity for SMP30 was detected. Meanwhile, 50 or 100 μg/L PSPC obviously attenuated the proportion of senescent cells.Moreover, D-gal administration for 24 and 48 hours decreased the level of SMP30 in HUVECs in contrast with the control group, and PSPC reversed this phenomenon and recovered the expression of SMP30. |
| Target gene: | NLRP3 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | NLRP3 |
| Target gene experiment: | Western blot//Immunofluorescence |
| Target gene description: | The results of western blot analysis showed that D-gal significantly increased the expression of NLRP3 in HUVECs as compared with that of vehicle control. On the contrary, PSPC administration depressed the amplification of NLRP3 induced by D-gal. A similar pattern of NLRP3 was observed for the immunofluorescence activities in the brachiocephalic artery of mice which were fed with HFD or HFD+PSPC. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: