| External factors: | Genistein |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | The effect of genistein on SA-β-gal activity was also observed and genistein was found to effectively suppress the expression of SA-β-gal in a dose-dependent manner.It was observed that treatment of HDFs with 40 and 80 mg/ml of genistein for 24 h decreased the number of early apoptotic cells (LR) significantly when compared to 18.1% in vehicle control (0 mg/ml) group.HDFs in G0/G1 phase was diminished in quantity upon different doses of genistein (20 mg/ml, 40 mg/ml, and 80 mg/ml), indicating a dose-dependent inhibition in cell cycle arrest by genistein. |
| Target gene: | P66SHC//FKHRL1 |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | P66SHC//FOXO3 |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | p66Shc and FKHRL1 were significantly up-regulated by UVB irradiation, and genisteintreatment of HDFs result edinadose-dependent decrease in their expression. The phosphory-lation of p66Shc and FKHRL1 were also down-regulated by different concentrations of genistein, which indicated genistein, as one of antioxidants, counteracts the UVB-induced oxidative stress at least in part by regulating the expression and activation of p66Shc and FKHRL1 protein. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: