| External factors: | TMZ |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Immunofluorescence staining//SAHF |
| Description: | TMZ is able to induce senescence in LN-229 and, to a lesser extent, in U87 MG cells. Senescence starts to become detectable 48 and 72 h after TMZ addition in LN-229 and U87 MG cells, respectively, and persisted over the whole post-incubation time period (up to 144 h). We also performed a cytochemical senescence detection assay using the chromogenic substrate 5-bromo-4-chloro-3-indoyl β-D-galactopyranoside (X-gal), which yields an insoluble blue compound when cleaved by β-gal. Further, we used another marker for senescence, the senescence-associated heterochromatic foci (SAHF), which are usually enriched for histone H3 methylated on lysine 9 (H3K9), which were not observed in non-treated but induced in TMZ-treated cells. |
| Target gene: | 3-MA |
| R-EF-Target gene: | -- |
| Official symbol(s): | 3-MA |
| Target gene experiment: | Flow cytometry |
| Target gene description: | The data shows that 3-MA completely abolished senescence after TMZ treatment. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: