| External factors: | Ceramide |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Cell morphological analysis |
| Description: | Young HDF were able to label 67.5 ± 9.5% of their nuclei by [3H]thymidine in response to serum stimulation. In contrast, senescent cells had a labeling index of <2% , cells grown in 10 μM C6-ceramide had a labeling index of 12 ± 5% ,and cells grown in 15 μM C6-ceramide had a labeling index of 2.5 ± 0.5% and morphologically resembled senescent cells. |
| Target gene: | AP1//RB |
| R-EF-Target gene: | --//-- |
| Official symbol(s): | JUN//RB |
| Target gene experiment: | Western blot |
| Target gene description: | Upon serum stimulation young HDF are able to respond by activation of AP-1 (lanes 1 and 2), whereas ceramide-treated cells (lanes 5 and 6) like senescent cells (lanes 7 and 8) were unable to respond by activation of AP-1. Remarkably, C6-ceramide in concentrations ranging from 3 to 20 μM was able to progressively inhibit Rb phosphorylation in young HDF in response to serum stimulation as indicated by Western blot analysis . In addition, C6-ceramide (15 μM) was able to induce complete Rb dephosphorylation by 20-24 h, thus mimicking senescent HDF. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: