| External factors: | MNNG |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Interestingly, at 100 μM of MNNG treatment,the HCT-116 cells showed a major change in their cell cycle arrest profile in which the G0/G1 phase arrest was significantly increased, and the G2/M phase arrest was decreased as compared to control cells.Our result showed that senescence-associated β-galactosidase staining increased in cells treated with 100 μM MNNG as compared to untreated cells. |
| Target gene: | APC |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | APC |
| Target gene experiment: | Western blot//Northern blot |
| Target gene description: | After 50 h of treatment with MNNG, the APC mRNA levels decreased, which paralleled with the decreased levels of APC protein. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: