| External factors: | Nitric oxide |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//qRT-PCR //ELISA |
| Description: | NO treated HeLa cells stained positive for SA-βgal activity, a hallmark of cellular senescence and the percentage of stained cells increased proportionally to the dose of SNP . The senescent state was further confirmed by recording the induction in the expression of p21, NF-κb and IL8 and reduction in the expression of proliferation cell nuclear antigen (PCNA)at RNA level. Additionally, the levels of IL8 and IL6 cytokines, a part of the senescence associated inflammatory phenotype were also significantly elevated after SNP or DETA. |
| Target gene: | ATM |
| R-EF-Target gene: | Activation |
| Official symbol(s): | ATM |
| Target gene experiment: | Cell viability assay |
| Target gene description: | Towards this, cells were treated with NO in the presence of a specific ATM kinase inhibitor, KU-55933 [46]. It is known that ATM kinase inhibition leads to an increase in ROS levels [44,45,47], which was observed when HeLa cells were treated with KU-55933, which increased further on NO treatment and was accompanied with significant loss of cell viability, as determined by a reduction in their proliferation as well as their metabolic activity . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: