| External factors: | Telomeric plasmid |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Gastric cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//MTT assay |
| Description: | Cells were transfected with telomeric plasmid pRST5. Twenty-four hours later, the cell growth was obviously inhibited . 0.5 mg mL 1 pRST5 plasmid transfection decreased cell viability by 27% and increased cell senescence by 62% ( p < 0.05). However, empty vector pBlueScript caused no obvious cell growth inhibition. |
| Target gene: | ATM//P53//TRF1//TRF2 |
| R-EF-Target gene: | --//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | ATM//P53//TERF1//TERF2 |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | Compared to control groups, cells transfected with telomeric plasmids showed an obvious increase of p53 protein level in a dose dependent manner.In addition, cells transfected with plasmid pRST5 displayed increase of TRF1 and TRF2 mRNA and protein level.20 mM wortmannin effectively inhibited the phosphorylation of ATM and the increase of p53, TRF1, and TRF2, suggesting that the upregulation of these proteins might be correlated with ATM activity. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: