| External factors: | photosensitizer 8-methoxy-psoralen and ultraviolet A radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Senescence of human primary fibroblasts was induced in 8-MOP-preincubated cells after irradiation with broad-spectrum UVA or monochromatic irradiation with 350 ±10 nm representing conditions that benefit ICL formation .Numbers of cells staining positive for γ-H2AX in primary human fibroblasts increased from 11% in untreated to 82% in PUVA-treated cells at 24 h after irradiation. |
| Target gene: | ATR |
| R-EF-Target gene: | Activation |
| Official symbol(s): | ATR |
| Target gene experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Target gene description: | The amount of SA-β-gal–positive cells was markedly reduced in the ATR siRNA-treated group compared with the ATM- or control siRNA-treated cells. From this, we conclude that depletion of ATR releases PUVA-treated fibroblasts from senescence. Notably, similar results were obtained in SV40-transformed WI26 VA4 fibroblasts, which also senesce after psoralen photoactivation .To test whether H2AX phosphorylation after PUVA depends on ATR, we ablated ATR expression in primary human fibroblasts by siRNA. ATR expression was almost abolished 24 h after transfection. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: