| External factors: | TBHP |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | SA-β-gal activity assay:SA-gal staining was significantly increased in the tBHPstimulated cells compared with the control cells. Flow cytometry:Flow cytometry was used to characterize the nature of cell cycle arrest. The cell cycle was arrested at the G0?G1 phase in the tBHP-stimulated cells (90.76% ± 6.34%) compared with the control cells. |
| Target gene: | BCL-2//BAX//STAT1//STAT3 |
| R-EF-Target gene: | Downregulation//Upregulation//Activation//Activation |
| Official symbol(s): | BCL-2//BAX//STAT1//STAT3 |
| Target gene experiment: | Western blot |
| Target gene description: | Bcl-2 protein expression decreased (p < 0.05) while Bax protein expression significantly increased (p < 0.01) in tBHP-induced cells compared with the control cells.Exposure of cells to 30 mol/L tBHP resulted in activation of STAT1 and STAT3 as manifested by an increase in STAT phosphorylation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: