| External factors: | JQ1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Flow cytometry//SA-β-gal activity assay |
| Description: | JQ1-treated MM.1S cells revealed a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1 ;Treatment with JQ1 resulted in pronounced cellular senescence by beta-galactosidase staining. |
| Target gene: | BET BROMODOMAIN |
| R-EF-Target gene: | Inhibition |
| Official symbol(s): | BET BROMODOMAIN |
| Target gene experiment: | Gene set enrichment analysis//Western blot//RT-PCR |
| Target gene description: | Gene sets defined by adjacency to Myc-binding motifs were in almost all cases significantly enriched in JQ1-suppressed genes we studied the consequence of JQ1 treatment on the expression of 230 cancer-related genes in a human MM cell line (MM.1S),Downregulation of MYC was further confirmed by RT-PCR and immunoblot. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: