Aging Overview

External factors

External factors: ZF1
Aging type: Prevent
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: Adipose,Bone marrow,Dental pulp,Perinatal tissue
Cell name: ASC
PMID: 31146388
Experiment: SA-β-gal activity assay//qRT-PCR
Description: Cells(culture passages 5th–7th) were treated for 72 h with ZF1 at the final concentrations of 0.01,10,and 20 μg/mL. Although 0.01 μg/mL ZF1 was ineffective,both 10 and 20 μg/mL ZF1 significantly reduced the number of senescent hASCs positively blue stained for SA β-gal (p < 0.05).In contrast, hASCs exposed to both 10 μg/mL and 20 μg/mL ZF1 resulted in a similar statistically significant increase in TERT transcription as compared with the control hASCs (SOLV).


Regulatory relationship

Target gene: BMI1//P53//P21
R-EF-Target gene: Upregulation//Downregulation//Downregulation
Official symbol(s): BMI1//P53//P21
Target gene experiment: qRT-PCR
Target gene description: We show that,in hASCs,ZF1 was also able to enhance the gene expression of BMI1, a pleiotropic transcriptional regulatoracting as a major repressor of senescence .On the contrary,in hBM-MSCs,hDP-MSCs,and hWJ-MSCs, he ZF-induced increase in TERT transcription was associated with a down-regulation in both TP53 (p53) and CDKN1A(p21) transcription.



Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.