External factors: | ZF1 |
Aging type: | Prevent |
Aging characteristic: |
Category: | Other |
Phenotype: | Aging |
Experimental category: | L |
Tissue type: | Adipose,Bone marrow,Dental pulp,Perinatal tissue |
Cell name: | ASC |
PMID: | 31146388 |
Experiment: | SA-β-gal activity assay//qRT-PCR |
Description: | Cells(culture passages 5th–7th) were treated for 72 h with ZF1 at the final concentrations of 0.01,10,and 20 μg/mL. Although 0.01 μg/mL ZF1 was ineffective,both 10 and 20 μg/mL ZF1 significantly reduced the number of senescent hASCs positively blue stained for SA β-gal (p < 0.05).In contrast, hASCs exposed to both 10 μg/mL and 20 μg/mL ZF1 resulted in a similar statistically significant increase in TERT transcription as compared with the control hASCs (SOLV). |
Target gene: | BMI1//P53//P21 |
R-EF-Target gene: | Upregulation//Downregulation//Downregulation |
Official symbol(s): | BMI1//P53//P21 |
Target gene experiment: | qRT-PCR |
Target gene description: | We show that,in hASCs,ZF1 was also able to enhance the gene expression of BMI1, a pleiotropic transcriptional regulatoracting as a major repressor of senescence .On the contrary,in hBM-MSCs,hDP-MSCs,and hWJ-MSCs, he ZF-induced increase in TERT transcription was associated with a down-regulation in both TP53 (p53) and CDKN1A(p21) transcription. |
Annotation: