| External factors: | Resveratrol/quercetin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Glioma |
| Experiment: | SA-β-gal activity assay |
| Description: | Senescence induction by Rsv plus Quer, measured by the activity of acidic β‐galactosidase, a biochemical marker for lysosome acidification,(40) was cooperative, since each treatment alone produced around 20% of SA‐β‐gal positive cells, whereas combination of Rsv and Quer induced staining in almost all cells. |
| Target gene: | CASPASE-3/7 |
| R-EF-Target gene: | Activation |
| Official symbol(s): | CASPASE-3/7 |
| Target gene experiment: | Annexin V binding assay//PI staining |
| Target gene description: | Rsv (50 μM) increased caspase 3/7 activity after 48 h of treatment at an intensity similar to 50 μM etoposide, used as a positive control. Quer (100 μM) was unable to induce caspase 3/7 even at 72 h of treatment. Neither 10 μM Res nor 25 μM Quer alone were able to induce caspase 3/7 activation; however, a strong activation was observed with the cotreatment, suggesting a synergistic effect of these compounds in caspase 3/7 activation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: