| External factors: | Ionizing radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Nasopharyngeal carcinoma |
| Experiment: | MTS assay//Cell cycle analysis//SA-β-gal activity assay |
| Description: | IR induced around 54% CNE2 cell senescence at 48 h,up to 79% at 72 h. Similar results were observed in U2OS cells. 6 Gy of IR induced 32% senescent cells at 48 h,up to 56% at 72 h ;The proliferation of CNE2-R cells was retarded compared to CNE2 cells, and more CNE2-R cells were arrested in G0/G1 phase than CNE2 cells.More β-Gal-positive cells were observed in CNE2-R cells than CNE2 cells. |
| Target gene: | CDC6 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | CDC6 |
| Target gene experiment: | Western blot |
| Target gene description: | We observed that IR steadily elevated CDC6 protein levels 24, 48, and 72 h after IR exposure, though the cell proliferation was retarded. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: