External factors: | Hecogenin |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Lung cancer |
Experiment: | Flow cytometry//SA-β-gal activity assay |
Description: | At 50 μM, there was a small decrease in cells at S phase and a small increase in cells at G0/G1 and G2/M . This effect was enhanced with increased concentrations of hecogenin acetate. At 75 μM, 74% of cells were induced to cell cycle arrest in G0/G1 while 84.3 % of cells were induced to G0/G1 cell cycle arrest by hecogenin acetate 100 μM;Control cells showed only few SA-β-Gal staining spots, in contrast to cells treated with hecogenin acetate 50 μM where clusters of senescent cells were evident. |
Target gene: | ERK1/2//MMP2 |
R-EF-Target gene: | --//-- |
Official symbol(s): | ERK1/2//MMP2 |
Target gene experiment: | Western blot |
Target gene description: | Hecogenin acetate completely abolished ERK 1/2 phosphorylation induced by H2O2.Hecogenin acetate was able to inhibit the increase in MMP-2 content caused by H2O2 treatment. |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: