| External factors: | AMG 232+radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Cancer |
| Experimental category: | L |
| Tissue type: | Lung,Colon,Melanoma,Mammary Gland |
| Cell name: | H460,A549,HCT116,A375,SJSA-1,MCF-7,H226 |
| PMID: | 26162687 |
| Experiment: | SA-β-gal activity assay |
| Description: | We first examined the activity of senescence-associated β-galactosidase (SA-β-Gal) and found that combined treatment of AMG 232 and radiation resulted in a significant increase in SA-β-gal positive cells compared with treatment with either radiation or AMG 232 alone in all cell lines tested. |
| Target gene: | FOXM1 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | FOXM1 |
| Target gene experiment: | qRT-PCR//Western blot//Immunofluorescence |
| Target gene description: | FoxM1 is a critical proproliferative transcription factor, and its inhibition leads to cellular senescence. Immunofluorescent imaging in A375 cells revealed that treatment with either AMG 232 or radiation alone at 48 hours resulted in a minor reduction in nuclear FoxM1 staining, whereas combination treatment resulted in a significant reduction in the number of FoxM1-positive cells. Furthermore, immunoblotting and RT-PCR analysis confirmed a reduction in protein and mRNA levels of FoxM1 following the combined treatment of AMG 232 and radiation in A375 . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: