| External factors: | X-ray |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | X-ray irradiation at these doses substantially induced heterochromatic focus formation and β-galactosidase expression,both of which are considered markers of cell senescence [13], in periosteal cells after 3 days.Quantitative analyses were performed for all of the oxidative stresses,a finding common to all stressors was that the percentage of cells in the G1/G0 phase decreased in a dose-dependent manner. |
| Target gene: | G-H2AX//P53//P21/CP1 |
| R-EF-Target gene: | Upregulation//Upregulation//Upregulation |
| Official symbol(s): | G-H2AX//P53//P21/CP1 |
| Target gene experiment: | Western blot |
| Target gene description: | After 2 h of x-ray irradiation or H2O2treatment, all of the oxidative stresses upregulated g-H2AX and p53 without appreciable changes in PCNA (when normalized by actin) in periosteal cells. In contrast, a cyclin-dependent kinase inhibitor, p21WAF1/cip1, was upregulated by x-ray. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: