| External factors: | Malignant ascitic fluids |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Ovarian cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | By doing so, we found that the percentage of cells in the S-phase of the cell cycle in cultures exposed to malignant ascitic fluids was decreased by 40 ± 2 % (p < 0.01) compared to their counterparts subjected to the control ascitic fluids. This effect coincided with an increased activity of SA-β-Gal, an indicator of cellular senescence, by 25 ± 5 % (p < 0.03) compared to the control group .At the same time, we found that cultures exposed to benign ascitic fluids contained ~10 ± 4 % prematurely senescent cells (no significant difference), whereas in cultures exposed to malignant ascitic fluids the subset of senescent cells increased to34 ± 4 % (p < 0.01). |
| Target gene: | GRO-1//HGF |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | CXCL1//HGF |
| Target gene experiment: | SA-β-gal activity assay |
| Target gene description: | We found that the concentrations of 3 of these factors, i.e. GRO-1, HGF and TGF-β1, differed in malignant versus benign ascitic fluids. The levels of these factors were uniformly higher in the malignant ascitic fluids.We found that the activity of SA-β-Gal in the HPMCs was increased when they were exposed for 72h to exogenous GRO-1. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: