| External factors: | tris-C60 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Interestingly, unlike hexa-C60, all doses of tris-C60 induced a significant increase (～12%) in cells arrested in G0/G1phase with fewer cells entering the S phase.No senescent cells were observed in the control samples.However, all doses of tris-C60(from 25 to 100 μg/ml) induced significant senescence at 24 and 48 h. Moreover, when tris-C60was removed by washing and cells incubated with fresh medium, the number of cells undergoing senescence was significantly reduced 24 and 48 h post-tris-C60treatment, suggesting that tris-C60 induced senescence was reversible. |
| Target gene: | HERC5//P16//P21//P53 |
| R-EF-Target gene: | Downregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | HERC5//P16//P21//P53 |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | A significant number of cell cycle related genes were altered by less than 2-fold in response to 100 μg/ml tris-C60 treatment at the 24 h time point. Surprisingly, a notable 5-fold decrease was observed in the expression level of HERC5, a cyclin E binding protein. These results were confirmed with Taqman RT-PCR using different concentrations of tris-C60.A significant and simultaneous decrease in the level of HERC5 protein was noted in the tris-C60treated cells, with no detectable levels at the 100 μg/ml dose.An increase in p16,p21 and total p53 protein expression levels was observed in the tris-C60 treated cells indicating the involvement of these key cell cycle regulatory proteins. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: