| External factors: | CO |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//qRT-PCR//ELISA |
| Description: | The pretreatment with CORM-A1 significantly alleviated the 5FU-elicited senescence of HUVECs. The CO treatment alone did not alter the basal senescence level of HUVECs, but reversed 5FU-induced endothelial senescence in a dose-dependent manner.5FU significantly increased PAI-1 and TNF-α expression, and the upregulation of these SASPs was attenuated by CO treatment. |
| Target gene: | HO-1//ENOS//SIRT1 |
| R-EF-Target gene: | Upregulation//Upregulation//Upregulation |
| Official symbol(s): | HMOX1//ENOS//SIRT1 |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | 5FU significantly decreased the HO-1 mRNA expression, whereas downregulation of HO-1 mRNA expression was enhanced by the CO treatment. 5FU significantly decreased eNOS and SIRT1 protein expression, whereas the downregulation of eNOS and SIRT1 protein expression was increased by the CORM-A1 treatment.The SIRT1 deficiency prevented CORM-A1 inhibition of 5FU-induced p21, PAI-1, TNF-α expression. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: