| External factors: | Homocysteine |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//qRT-PCR//Flow cytometry |
| Description: | As expected, homocysteine could accelerate EC senescence when compared with standard conditions. Interestingly, homocysteine-increased p16, p21, and p53 expressions as well as SA-β-Gal positive cells, apoptosis cells, and cell cycle . |
| Target gene: | HTERT |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | HTERT |
| Target gene experiment: | Western blot |
| Target gene description: | Indeed, homocysteine also accelerated the reduction in hTERT mRNA and protein expression from P6 to P10. In addition, homocysteine decreased the expression of hTERT in a dose-dependent manner in P4-5 HUVECs. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: