| External factors: | Ionizing radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | We confirmed that incidence of the senescence-like phenotype increased after exposure to 4-Gy IR. Like in senescent cells, cells began to exhibit an enlarged and fl attened cell shape and increased their SA- β -gal activity up to 22% and 39%, respectively, at 2 and 4 Gy of IR. |
| Target gene: | IGFBP5 |
| R-EF-Target gene: | -- |
| Official symbol(s): | IGFBP5 |
| Target gene experiment: | MTT assay//SA-β-gal activity assay//Tube formation assay |
| Target gene description: | IGFBP5-downregulated cells were increased in cell proliferation compared with the control cells during irradiation. Based on SA- β -gal staining, IR increased SA- β -gal activity in control virus-transduced cells but not in IGFBP5 miRNA transduced cells. By tube formation assay, we observed that decrease in endothelial cell tube formation by irradiation was recovered by IGFBP5 down-regulation.These results demonstrate that IR-induced IGFBP5 is associated with radiation-enhanced cellular senescence and IGFBP5 downregulation can regulate radiation damage. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: