| External factors: | HDAC inhibitor(sodium butyrate(NaBu) and valproic acid(VPA)) |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | We extended the treatment period up to 7 days, where we observed tha MSCs acquired a senescent phenotype based on SA-β-gal staining. |
| Target gene: | JMJD3//P16//SUZ12//BMI1//EZH2//C-MYC |
| R-EF-Target gene: | Upregulation//Upregulation//Downregulation//Downregulation//Downregulation//Downregulation |
| Official symbol(s): | KDM6B//P16//SUZ12//BMI1//EZH2//C-MYC |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | hUCB- and hADMSCs showed decreased expression of c-MYC, BMI1 and SUZ12. JMJD3 was upregulated by 1-day treatment of HDAC inhibitors, and its expression level peaked at 3 days of treatment. p16INK4Awas slightly increased from 3 days of treatment of HDAC inhibitors. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: