| External factors: | Rapamycin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Werner syndrome |
| Experiment: | Western blot//Immunochemical staining |
| Description: | At the end of 42 days, growth rates reached 1.3 population doubling (PD) per week for WRN knockdown cells with rapamycin compared with 0.4 PD per week without rapamycin. Moreover, long-term rapamycin treatment restored normal nuclear morphology of WRN knockdown fibroblasts. |
| Target gene: | LC3-II//P62 |
| R-EF-Target gene: | Upregulation//Downregulation |
| Official symbol(s): | MAP1LC3A//P62 |
| Target gene experiment: | Western blot |
| Target gene description: | Rapamycin induced increases in LC3-II and decreases in p62, consistent with enhanced degradation of autophagosomes. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: