| External factors: | UNC2025 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Glioblastoma |
| Experiment: | Cell Cycle analysis//Flow cytometry//SA-β-gal activity assay//BrdU assay//PI staining |
| Description: | The amount of BrdU incorporation significantly decreased following UNC2025 treatment, indicating decreased proliferation in SF188 and U251 cells, while only a trend of decreased proliferation was observed in A172;treatment of A172, SF188, and U251 cells with UNC2025 induced beta-galactosidase activity (blue staining), a prominent marker of senescence.[25] .At a molecular level, p16 and p21 are markers of senescence [24]. |
| Target gene: | MERTK |
| R-EF-Target gene: | -- |
| Official symbol(s): | MERTK |
| Target gene experiment: | Densitometry analysis//Western blot |
| Target gene description: | Afterone hour of UNC2025 treatment, MERTK phosphorylationwas reducedin all three celllines and in a dosedependentmanner. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: