Aging Overview

External factors

External factors: UNC2025
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Glioblastoma
Experimental category: L
Tissue type: --
Cell name: A172,SF188,U251
PMID: 27783662
Experiment: Cell Cycle analysis//Flow cytometry//SA-β-gal activity assay//BrdU assay//PI staining
Description: The amount of BrdU incorporation significantly decreased following UNC2025 treatment, indicating decreased proliferation in SF188 and U251 cells, while only a trend of decreased proliferation was observed in A172;treatment of A172, SF188, and U251 cells with UNC2025 induced beta-galactosidase activity (blue staining), a prominent marker of senescence.[25] .At a molecular level, p16 and p21 are markers of senescence [24].


Regulatory relationship

Target gene: MERTK
R-EF-Target gene: --
Official symbol(s): MERTK
Target gene experiment: Densitometry analysis//Western blot
Target gene description: Afterone hour of UNC2025 treatment, MERTK phosphorylationwas reducedin all three celllines and in a dosedependentmanner.

Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.