| External factors: | 21% O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | Growth curve assay//SA-β-gal activity assay |
| Description: | The recorded cell counts demonstrated increased cell proliferation under 21% O2 compared to 5% O2with a major significant gap after 72 h.The percentage of blue cells is significantly higher in old fibroblasts grown under 21% O2 compared to those grown under 5% O2. |
| Target gene: | MIR-181A//MMP1//COL1A1 |
| R-EF-Target gene: | Downregulation//Upregulation//Downregulation |
| Official symbol(s): | MIR-181A//MMP1//COL1A1 |
| Target gene experiment: | qPCR |
| Target gene description: | Our results show that in comparing cells cultured under 21% O2 with those cultured under 5% O2, we observe downregulation of miR-181a in young cells while an opposite highly significant upregulation was observed in old cells.The results show that there is a significant activation of MMP1 mRNA expression in both young and old cells, indicating that HDF cultured under 21% O2are more aged with respect to those cultured under 5% O2. The same statement is valid also for mRNA expression of COL1A1 which is significantly downregulated under 21% O2 in old cells compared to 5% O2, an indication that cells are more aged. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: