| External factors: | Rapamycin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: | Rapamycin was added to REF cells at passage 7 , and the cells were grown for 3 additional passages in rapamycin-containing media. Afterwards, rapamycin was removed, and during the subsequent passages the cells were becoming smaller in size,thereby reflecting a gradual restoration of the non-senescent phenotype as evidenced by Giemsa staining.The population lost such senescence markers as SA-β-Gal staining and restored the lost ability to proliferate as evidenced by an increase of S-phase cells. |
| Target gene: | MTERT |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | MTERT |
| Target gene experiment: | RT-PCR |
| Target gene description: | Interestingly, transcription of telomerase gene (mtert) is additionally augmented in rapamycin-derived cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: