| External factors: | FK866 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Retinal disease |
| Experiment: | SA-β-gal activity assay//qPCR//Western?blot |
| Description: | β-galactosidase staining and related quantification revealed a time-dependent increase in the number of β-gal-positive cells in FK866-treated cultures .Similarly, the expression of p21Waf/Cip1, p16INK4a, ApoJ and CTGF was increased and SIRT1 expression and activity decreased time-dependently. |
| Target gene: | NAD+//SIRT1 |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | NAD//SIRT1 |
| Target gene experiment: | SA-β-gal activity assay//qPCR//Western?blot |
| Target gene description: | Congruent with β-galactosidase staining, the expression of each of these markers was increased in association with increasing concentrations of FK866 and the associated decline in NAD+ . Additionally, the expression and activity of SIRT1 was dose-dependently decreased . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: