| External factors: | Galangin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity//MTT assay//Western blot |
| Description: | HS68 cells were pretreated with galangin (30 lM) for 1 hour followed by incubation with H2O2 (200 lM) for 24 hours prior to SA-β-gal staining. We found that H2O2 significantly increased the number of senescent cells (green) as compared with the control. These data demonstrate that galangin can significantly inhibit H2O2-induced cell senescence .The MTT assay results showed that galangin dosedependently increased the viability of H2O2-induced HS68 cells. |
| Target gene: | NF-KB |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | NF-KB |
| Target gene experiment: | Western blot |
| Target gene description: | The data demonstrated that galangin reduced the levels of H2O2-induced inflammation-related proteins, IL6, TNF-a, IL-1b, and p-NF-jB p65 . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: