| External factors: | GATA4 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//BrdU assay |
| Description: | Ectopic expression of GATA4 induced senescence in human foreskin fibroblasts and IMR-90 fibroblasts ,as shown by increased senescence-associated β-galactosidase (SA-β-Gal) activity and decreased 5-bromo-2′-deoxyuridine (BrdU) incorporation. More important, depletion of GATA4 with stably expressed short hairpin RNAs (shRNAs) partially decreased IR-induced SA-β-Gal activity and delayed replicative senescence. |
| Target gene: | NF-κB |
| R-EF-Target gene: | Activation |
| Official symbol(s): | NFKB1 |
| Target gene experiment: | Immunocytochemistry//Western blot |
| Target gene description: | GATA4 expression triggered NF-κB activation, and GATA4 depletion inhibited NF-κB activation during senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: