| External factors: | AngII |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | After coincubation with Ang II (200 nmol/L) for 24 h, the number of SA-β-Gal-positive or G0/G1 resting cells increased significantly. |
| Target gene: | NOX2//NOX4//NOX5 |
| R-EF-Target gene: | Upregulation//Upregulation//Upregulation |
| Official symbol(s): | CYBB//NOX4//NOX5 |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | Results in late EPCs treated with Ang II, revealed that differential expression of NOX mRNA existed. Within 5 kinds of NOX mRNA, NOX2, NOX4 and NOX5 mRNA levels were significantly increased.Similarly, in Western blot,Ang II (200nmol/L) resulted in maximum increase in NOX5 protein level at 3h versus NOX2 and NOX4 at 12h. However, siRNA targeting NOX2 or NOX4 yielded a significant decrease in SA-β-Gal positive or G0/G1 resting cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: