| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Cardiovascular disease |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | We treated primary human endothelial cells in passages 2–3 with 50 μM H2O2 every second day for two weeks. As expected and previously published , this treatment induces senescence as evaluated by senescence associated (SA)-β-Galactosidase staining. we also quantified a known senescence marker, the cell cycle inhibitor p21 and its nuclear localization by immunostaining. H2O2increases the percentage of cells positive for nuclear p21 as well as the total protein levels of p21. |
| Target gene: | NOX4//TRX-1//CATHEPSIN D |
| R-EF-Target gene: | Upregulation//Downregulation//Activation |
| Official symbol(s): | NOX4//TRX-1//CATHEPSIN D |
| Target gene experiment: | qRT-PCR//Western blot//ELISA |
| Target gene description: | Relative NOX4 expression and NOX4 protein levels are increased under H2O2treatment. After two weeks treatment with H2O2,Trx-1 protein levels are decreased.Two weeks treatment with H2O2 induces over-activation of Cathepsin D. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: