| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Immunofluorescence |
| Description: | We found that p16 overexpression or H2O2 treatment alone induced senescence in WI-38 cells and the combination of both resulted in an additive effect. Meanwhile, the senescence-associated heterochromatin foci (SAHF) assay confirmed the observations in senescence cell staining. Both 3MeK9H3 and HMGA1, two classic markers of SAHF, localized to the specific heterochromatic foci in cells transfected with p16 and treated with H2O2. |
| Target gene: | P16 |
| R-EF-Target gene: | -- |
| Official symbol(s): | P16 |
| Target gene experiment: | Co-IP |
| Target gene description: | Consistent with the observations in 293T cells, our CoIP experiments showed that the level of p16 phosphorylation and the association of CDK4 with p16 increased upon the H2O2 treatment in WI-38 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: