Aging Overview

External factors

External factors: TBK1-II
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: HL
Tissue type: Mammary Gland
Cell name: HCC1954,SK-BR-3,Neu monolayer cell
PMID: 24487029
Experiment: SA-β-gal activity assay//Flow cytometry
Description: Remarkably, large, β-galactosidase-positive cells were observed in ~20% of lenti-shRNATbk1 transduced, and TBK1-II treated cultures .Strikingly, TBK1-II treatment (2 μM) virtually eliminated accumulation of cells in all phases and dramatically increased the percentage of cells with >4N chromosomes (M3 gate). This effect was also observed by forward- and side-scattering flow cytometry analysis.


Regulatory relationship

Target gene: P16//P65-NFκB
R-EF-Target gene: Activation//--
Official symbol(s): P16//P65-NFκB
Target gene experiment: Western blot
Target gene description: The NF-κB family proteins, RelA (p65), c-Rel, RelB, p50, and p52, bind to DNA as dimers, the most common being a p65-p50 heterodimer (43). P65-NFκB activity is induced by phosphorylation of serine536 (44). In untreated HER2+ BC cells, p65-NFκB was highly phosphorylated on serine536. Importantly, TBK1-II treatment of mouse and human HER2+ BC cells dramatically suppressed serine536-phosphorylation, hence activity of p65-NFκB. In addition, expression of the pro-senescence cyclin-dependent kinase inhibitor p16INK4A (45) was dramatically induced in both mouse and human HER2+ BC cells.

Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.