| External factors: | Nicotinamide |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | When treating with nicotinamide alone, the percentage of SA-β-gal-positive cells increased to 12.3 ± 2.5%; after treating with H2O2, nicotinamide significantly counteracted DECM-mediated anti-senescent effects by raising the percentage of SA-β-gal-positive cells to 57.2 ± 5.5% . |
| Target gene: | P16//SIRT1//P38 |
| R-EF-Target gene: | Upregulation//Downregulation//-- |
| Official symbol(s): | P16//SIRT1//P38 |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | The treatment with nicotinamide significantly increased p16INK4αin the H2O2-treated cells by 52.4% at the mRNA level and by 4.2-fold at the protein level compared with the untreated cells.The nicotinamide supplement also decreased SIRT1 in the H2O2-treated cells by 22.8% at the mRNA level and by 68.9% at the protein level .The western blot assay showed that exposure to nicotinamide and H2O2 increased phosphorylation of p38 by 4.2-fold compared with the control cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: