Aging Overview

External factors

External factors: Nicotinamide
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: L
Tissue type: --
Cell name: UC-MSC
PMID: 28107614
Experiment: SA-β-gal activity assay
Description: When treating with nicotinamide alone, the percentage of SA-β-gal-positive cells increased to 12.3 ± 2.5%; after treating with H2O2, nicotinamide significantly counteracted DECM-mediated anti-senescent effects by raising the percentage of SA-β-gal-positive cells to 57.2 ± 5.5% .


Regulatory relationship

Target gene: P16//SIRT1//P38
R-EF-Target gene: Upregulation//Downregulation//--
Official symbol(s): P16//SIRT1//P38
Target gene experiment: Western blot//RT-PCR
Target gene description: The treatment with nicotinamide significantly increased p16INK4αin the H2O2-treated cells by 52.4% at the mRNA level and by 4.2-fold at the protein level compared with the untreated cells.The nicotinamide supplement also decreased SIRT1 in the H2O2-treated cells by 22.8% at the mRNA level and by 68.9% at the protein level .The western blot assay showed that exposure to nicotinamide and H2O2 increased phosphorylation of p38 by 4.2-fold compared with the control cells.

Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.