| External factors: | Heat shock |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis//Western blot//DAPI staining |
| Description: | Inhibition of proliferation was confirmed by cell staining with antibodies to Ki-67, a marker of proliferative activity. Seventy-two hours after heat shock, only a few cells were Ki-67-positive. Cell cycle analysis by flow cytometry revealed that heated cells were arrested mostly in G2/M phase.The modified morphology of MSCs subjected to HS: they are enlarged,more flattened, and express X-gal. Three days after HS, the number of SA-β-gal-positive cells increased to about 70 %. HS induced expression of p21 protein, a cell cycle inhibitor and major regulator of the senescence program. |
| Target gene: | P21 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P21 |
| Target gene experiment: | Western blot//PCR |
| Target gene description: | HS induced expression of p21 protein, a cell cycle inhibitor and major regulator of the senescence program. Four hours after recovery, p21 upregulation was obvious both at mRNA and protein levels. During 72 h after HS, the p21 expression gradually increases up to three times. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: