| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: | H2O2 treatment was found to lead to development of senescent-like morphology: cells become enlarged, flattened,and heterogeneous. senescent cells demonstrated SA-β-Gal staining which increased gradually,and the most remarkable effect was reached at 7 days after treatment: more 95% H2O2-treated cells were SA-β-Gal positive .The analysis of the cell cycle phase distribution in hMESCs showed that a pulse H2O2 treatment led to the arrest in all of the cycle phases . |
| Target gene: | P21 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P21 |
| Target gene experiment: | Flow cytometry//Western blot |
| Target gene description: | 200uM H2O2 promoted a significant elevation in protein expression of p21 in 7 h after treatment. it has been reported that, in bone marrow-derived mesenchymal stem cells exposed to sublethal doses of H2O2,a rapid decrease of proliferation rate was detected within 3 days and correlated with G1 phase arrest of the cell cycle when p21 was accumulated at the same time. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: