| External factors: | MLN4924 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Cancer |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Indeed, all three lines of cancer cells after MLN4924 treatment at 0.1 μM concentration demonstrated an enlarged and flattened shape, a reminiscence of senescence phenotype.SA-β-Gal staining showed that ～50% of HCT116 cells were stained positively compared with only ～1% cells with positive staining in the DMSO control group . |
| Target gene: | P21 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P21 |
| Target gene experiment: | Western blot |
| Target gene description: | P21 was accumulated in a dose- and time-dependent manner in all three lines of cancer cells treated with MLN4924.P21, on accumulation by MLN4924, remained elevated in HCT116 cells or even continued to accumulate in H1299 cells after drug removal. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: