| External factors: | AMG 900+HDACI |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Prostate cancer |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Treated cells displayed a flattened morphology compared to untreated controls, and some treated DU-145 and PC3 cells showed cytoplasmic vacuoles and granularity.Consistent with our previously observed morphological transformations, DU-145 and PC3 cells treated with combinations of AMG 900 and HDACIs had increased SA β-galactosidase staining. LNCaP cells had a baseline level of blue SA β-galactosidase staining in untreated controls; combination treatment of LNCaP cells with AMG 900 and HDACIs resulted in increased SA β-galactosidase staining. |
| Target gene: | P21 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P21 |
| Target gene experiment: | Western blot |
| Target gene description: | Combination treatment further increased p21 levels, suggesting increased cellular senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: