External factors: | MLN4924 |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Lymphoma |
Experiment: | PI staining//FACS analysis //Cell morphological analysis//SA-β-gal activity assay |
Description: | A prominent G2-M cell-cycle arrest was observed in all treated cell lines (Raji, U937,SU-DHL-4 and Toledo) in a dose-dependent manner;An increase in cell size with flattened shape, a characteristic of senescence, was observed in Raji and U937 cells;the expression of SA-β-gal was determined by SA-β-gal staining in MLN4924-treated cells,a substantial proportion of MLN4924-treated cells were positively stained. |
Target gene: | P21//P27 |
R-EF-Target gene: | --//-- |
Official symbol(s): | P21//P27 |
Target gene experiment: | Western blot |
Target gene description: | MLN4924 significantly delayed p21/p27 turnover and extended the half-life of p21/p27 in both Raji and U937 cells.In contrast, MLN4924 had little effect on the transactivation of p21/p27 . |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: