| External factors: | Argentatin B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | SA-β-gal activity assay:After incubation with argentatin B for 72 h, both cell lines exhibited phenotypic changes that resemble those observed in cells undergoing senescence, such as flattened morphology and enlarged cell size.When tested for senescence associated-β-galactosidase activity, a proportion of 43% HCT-15, and 66% PC-3 cells showed a positive staining, compared with 2% of untreated controls. These findings suggest that argentatin B inhibits cell proliferation by inducing senescence. |
| Target gene: | P21//P27 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | P21//P27 |
| Target gene experiment: | Western blot |
| Target gene description: | Treatment with argentatin B induced an increment of both, p21 and p27 in PC-3 cells after 48 h incubation, and it was persistent for at least 72 h after treatment. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: