| External factors: | Oxidative stress |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Telomere length assay//Cell morphological analysis |
| Description: | Young MSCs incubated with sub-lethal doses of H2O2(100–150 μM) showed characteristic features of senescence concerning morphology and positive β-galactosidase staining . relative shortening referred to the original telomere length. Relative telomere loss was 2.91% per cpd (SD 0.43%) under chronic stress and 1.84% per cpd (SD 0.23%) in the control group. |
| Target gene: | P21//TRF1//TRF2//SIRT1//XRCC5 |
| R-EF-Target gene: | Upregulation//Downregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | P21//TERF1//TERF2//SIRT1//XRCC5 |
| Target gene experiment: | qRT-PCR |
| Target gene description: | In young MSCs, H2O2treatment caused a significant elevation in p21 expression after 1 h (2-fold of initial level) prior to a decrease to initial levels after 3 h. Significant p21 elevation up to a 4-fold rise could be detected in old MSCs 1 and 3 h after treatment.In young MSCs, TRF1 showed a significant decline in mRNA expression after 3, 6, 12 and 24 h. Senescent cells produced significantly less TRF1 after 6, 12 and 24 h. TRF2 expression in young and old cells was up-regulated significantly after 3 h and came down to initial ranks after 24 h。XRCC5 expression showed a slight increase in both groups witha maximum rise to the 1.5-fold in old cells. Oxidative stress caused a significant increase of SIRT1 gene expression after 1, 3, and 6 h in young MSCs. With a 2-fold increase maximum expression was found after 1 h and declined slowly to a level not significantly. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: