| External factors: | Nutlin-3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Sarcoma |
| Experiment: | SA-β-gal activity assay |
| Description: | After a 4-d exposure to nutlin-3 at a dose (2 lM) that had hardly exerted any apoptosis-inducing effect, CADO-ES-1 cells were stained for SA-β-Gal activity, the most widely used marker for cellular senescence. Nutlin-3-treated cells showed clear signs of senescence, whereas untreated cells did not. |
| Target gene: | P53 |
| R-EF-Target gene: | Activation |
| Official symbol(s): | P53 |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | Immunoblot detection revealed a rise of p53 after nutlin-3 exposure in the ES cell lines with wt-p53.To confirm the p53-activating effect of nutlin-3, we determined the gene expression of three key transcriptional targets of p5326 MDM2, p21 and PUMA by real-time RT-PCR.Nutlin-3 induced concentration-dependent increases in mRNA levels in all three cases in the wt-p53 cell lines, but not in the mt-p53 one. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: